![]() Plasmid DNA loaded chitosan nanoparticles for nasal mucosal immunization against hepatitis B. These in vivo findings demonstrate the superior stability of PEGylated polyplexes in physiological milieu and provide important insight on how cationic polymers could be optimized for DNA vaccine delivery. By day 4, co-localization of polyplexes with APCs was observed at the injection site regardless of polymer structure, whereas small amounts of polyplexes were found in the draining lymph nodes. PEGylated polyplexes also distributed more broadly among dermal fibroblasts and allowed greater interaction with antigen-presenting cells (APCs) (dendritic cells and macrophages) starting at around 24 h post-injection. The PEGylated polyplexes had a significantly wider distribution in the tissue than the nonPEGylated polyplexes. However, naked plasmid level dropped to below detection limit after 24 h, whereas polyplexes persisted for up to 2 weeks. ![]() Live animal imaging revealed that naked plasmid dispersed quickly in the skin of mice after injection and had a wider distribution than any of the three types of polyplexes. At day 4, however, the polyplexes appeared to result in more transfected skin cells than naked plasmid. Comparable numbers of luciferase expressing cells were observed in the skin of mice receiving naked plasmid or polyplexes one day after transfection. We analyzed transgene expression (luciferase) and the local tissue distribution of the labeled plasmid at the injection site at various time points (from hours to days). To better understand the details of DNA vaccine delivery in vivo, we prepared polymer/ DNA complexes using three structurally distinct cationic polymers and fluorescently labeled plasmid DNA and injected them intradermally into mice. Noelle Zhong, Xiao Panus, David Han, Wenqing Ji, Weihang Wang, ChunĭNA vaccination using cationic polymers as carriers has the potential to be a very powerful method of immunotherapy, but typical immune responses generated have been less than robust. Recipient Outstanding Radio Personality, Time Magazine, 1946, award, Organization for Christians and Jews, 1949, Outstanding Production and Popular Series award, Academy television Arts and Sciences, 1949, award, Sheltered Workshops American, 1950, Best Supporting Actress award, Radio Life Magazine, 1950, Directors Choice award, Redbook Magazine, 1952, Lifetime Contribution to Radio Broadcasting, Golden Mike Sperdvak-Kane, 1989, Lifetime Achievement Trophy, New Jersey Radio Broadcasters, 1992, Double Gold award, Corporation for Public Broadcasting, 1992, Silver Reel award, International Federation Indiana Producers, 1997, Ray Bradbury Creativity award, Woodbury University.A., 1998, 27 national and international awards, Special Certified of Commendation for Cultural Contributions, Los Angeles City Council and Mayor, 2003, Silver award, International Radio Festival, 2003, 5 Gold World Globe awards, 1989-2002, 5 Silver World Globe awards, 1989-2002, 2 Bronze World Globe award, 9 finalist awards, 1989-1994, award for Producing Quality Radio Drama for 20 years, Pacific Pioneer Broadcasters, 2005.Transgene expression and local tissue distribution of naked and polymer-condensed plasmid DNA after intradermal administration in mice Children: Teresa Dickinson, Patricia Wynn, Robert Marshall Junior. Married Robert Sinskey, Aug(divorced 1968). Member of Screen Actors Guild, American Federation of television and Radio Artists, Pacific Pioneer Broadcasters (former board directors), Actors Equity Association.Īvocations: walking, history, genealogy, archaeology. Peggy Webber has been listed as a notable Actress, producer, director, writer by Marquis Who's Who.įounder, president California Artists Radio Theatre Non-profit Corporation, artistic director. In 1979, she played many characters on Sears Radio Theater. One of her most notable roles came in the 1956 movie The Wrong Manitoba, directed by Alfred Hitchcock. Her screen debut came in the 1946 film Her Adventurous Night. Student, University Southern California, Los Angeles, 1942-1944 Daughter of Mathew Edward and Margaret Ann (Pierce) Weber. Webber, Peggy was born on Septemin Laredo, Texas, United States. Founder, president California Artists Radio Theatre Non-profit Corporation, artistic director Member of Screen Actors Guild, American Federation of television and Radio Artists, Pacific Pioneer Broadcasters (former board directors), Actors Equity Association. Peggy Webber, American actress, radio director, radio producer, writer.
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